What type of buffer is recommended for dialysis?
A. The best buffer to use is 10 mM Tris pH 8.0, 2mM MgCl2, 4% sucrose; this buffer enables one to concentrate the virus to approximately 1 x1013 VP/mL (viral particle/mL) without precipitation and provides very good stability for long-term storage and shipping. If the virus is to be used for animal studies, a buffer with glycerol should not be used since it is difficult to inject. PBS buffers can also be used but do not provide very good viral stability and should be avoided if the virus has to be concentrated; the particles will likely precipitate due to the low pH (~7) involved. Using a PBS buffer will enable concentration of the virus up to approximately 5 x 1011 VP/mL without precipitation. Viruses in PBS buffer will also be severely affected by repeated freeze/thaw cycles. For these reasons we recommend the Tris buffer over PBS for all applications. Reference: Nyberg-Hoffman, C. and E. Aguilar-Cordova (1999). “Instability of adenoviral vectors during transport and its implication
