What transfection method should I use with the SureSilencing™ shRNA plasmids?
If you have previously developed a highly efficient transfection method for your cell line, you may use that method to transfect the SureSilencing™ shRNA plasmids. If you have never optimized transfection of your cell line of interest before, we recommend using SABiosciences’ SureFECT Transfection Reagent (SA-01). SureFECT is incredibly versatile. Its used in a reverse transfection protocol that yields exceptionally high transfection efficiencies in most cell lines, for both plasmids and siRNA molecules. A complete description of this reagent can be found at the following web address: http://www.sabiosciences.com/reversetransfection.php As mentioned in the DELIVERY OF siRNA/shRNA FAQs, it is important to optimize transfection conditions for your transfection reagent and cell line of interest. In order to carry out process optimization studies, either use the GFP-based SureSilencing™ shRNA plasmids or another plasmid system that has an easily screened marker that allows you to count cel
Related Questions
- How can I use the SureSilencing™ shRNA plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?
- Can I use fluorescence microscopy to assess the transfection efficiency of the SureSilencing™ shRNA plasmids with GFP in my model cell line of interest?
- What transfection method should I use with the SureSilencing™ shRNA plasmids?
