What to the green and red colors within the preview window mean?
A. The Preview Window allows users to see the results of segmentation prior to quantifying an image or images. The purpose of this window is to be certain that the proper settings have been established so that ImaGene removes contamination, such as dust, properly. ImaGene uses patent-pending statistical methods to determine whether individual pixels belong to the signal or background regions. Green represents pixels that will be used in calculating a background value. Red represent pixels that will be used in calculating a signal value. Pixels that are neither red nor green are ignored and thus not used in the calculation of mean, median, etc values. Ultimately, the Preview Window helps user establish optimal parameters ensuring high quality, consistent data.