What to look for in the Agilent RNA Quality Check result?
For information about the RNA Ladder we are using. Check here : http://www.ambion.com/techlib/spec/sp_7152.pdf For total RNA: RNA quality: the ratio for rRNA 28s/18s is a indication of RNA quality. The idea ratio is 2, a low ratio is a indication of degradation of the total RNA. On the gel image and the graph, the 28s and 18s should show up as 2 sharp bands. RNA concentration: Agilent RNA quality check can give you an estimate of the RNA concentration. If concentration is what you concerned about, double check it on a spec. For mRNA: rRNA contamination: the percentage of rRNA in your sample. The idea number is <5. RNA concentration: Agilent RNA quality check can give you an estimate of the RNA concentration.
