What technologies effectively determine the classification of protein electrophoresis results?
Have there been any advancements which improve accuracy? The most common life sciences research methodology used to separate proteins from cellular lysates is protein electrophoresis. This method reveals individual protein expression levels relative to the other proteins in the lysate. The conventional method employed to quantify these probed for protein signals, resulting from antibody interactions with targeted epitopes of proteins bound to nitrocellulose or polyvinylidene difluoride (PVDF) membranes, was through the use of horseradish-peroxidase (HRP)-conjugated secondary antibodies. Through a chemiluminescent reaction, a light signal is emitted from the double antibody-protein complex, and after exposure to autoradiographic film, the signal can be visualized. However, this system is not very reliable, in that exposure times and other variables that exist in the experimental process can alter the outcome, with the data that is produced regarded as qualitative in nature, not quantita
