What technique is used at the facility to label the cDNA?
Several labeling strategies are currently in use at the facility. We most commonly use an Agilent Technologies two-colour direct-labeling protocol which requires a starting amount of 10-20 µg of total RNA per channel. Please contact us to discuss which protocol best suits your needs. Agilent One-Colour and Two-Colour labeling protocols can be found at www.agilent.com . (Select “Manuals & Guides”, then “Life Sciences and Chemical Analysis Solutions”.