What technique do I use for the petri dish?
• There are two techniques that we have learned thus far. • The first is a streak method, which can be seen by clicking here. This is by far a simple technique in which involves a simple zigzag pattern of inoculation. This is obtained by lightly touching the instrument used to obtain the sample to the surface of the agar and applying a back and forth motion to spread the sample. The second is the quadrant method. This uses the same pattern as above, except that one does this pattern in the quadrants. An example of this method can be seen by clicking here. One starts in one quadrant and streaks the sample. Flame the loop, then (cool the loop an a piece of the agar at the edge) streak the second quadrant by overlapping the first streak. Flame the loop, then streak the third quadrant by overlapping the second. Finally, Flame the loop and then streak the final quadrant by overlapping the third.
