What target preparation is used for expression arrays and why?
3’ arrays: The NuGEN Ovation target preparation system is used if RNA concentration is 5 ng/ul or higher. For lower recovery targets, the NuGEN Pico Ovation is generally employed but special procedures are inline to perform a NuGEN Ovation. Exon and Gene arrays: The NuGEN WT-Ovation system combined with the WT-Ovation Exon Module has been successful in the facility for generating high quality, reproducible data endpoints. The Facility staff have extensively tested target preparation systems compatible with Affymetrix GeneChip technologies and have found the NuGEN product lines to be most sensitive and requiring very little input material. Can I still request the standard Affymetrix -Eberwine target preparation? Yes, if requested. It is recommended if you want to generate new data that can be directly compared to a previous data set that used the “Eberwine” approach. The facility will only offer this target preparation for those who specifically request it.
