What strain(s) do you recommend as hosts for the pMAL vectors?
The strain we have used most frequently is TB1, #E4122S, which is JM83 hsdR-. There is nothing special about it with respect to the pMAL system, but it has given the best all-around results when considering plasmid stability, expression and purification. We have used a number of other strains successfully for certain proteins. We also use other strains in response to particular problem (for example, see 2.1). One can start with TB1, or one can use whatever competent cells are readily available and then try TB1 or another strain if a problem with expression or purification develops.
