What strain should I use to generate F1 mice for expression analysis?
Founder mice can, in general, be bred to any desired strain. We recommend mating wild-type mice, not non-transgenic littermates to founders initially to begin establishing the line and generating F1 for expression analysis. Mixed B6:SJL founders can be kept on a B6SJLF1 background or crossed with C57BL/6 mice if interested in backcrossing the transgene onto a pure background strain.
