WHAT STEPS ARE INCLUDED IN THE COMBIMATRIX PROBE DESIGN ALGORITHM?
You can provide a file with GenBank accession numbers or nucleotide sequences that correspond to genes of interest. Our probe design software applies a set of proprietary algorithms that is based on the generally accepted probe selection rules, and trained from experimental data obtained with CustomArray chips. This software will perform several subsequent iterations to select probes that are unique within the defined gene set, are located in the specified part of genes, have Tm and length within the specified range, do not form stable secondary structure, and do not carry repeat sequences.