What standards does the PHS Policy require our institution to follow in conducting survival rodent surgery, and how do they differ from those applicable to other species?
The PHS Policy requires that the recommendations of the Guide for the Care and Use of Laboratory Animals (Guide) (NRC, 1985) be adhered to regarding survival surgical procedures and the environments in which they are conducted. The Guide presents slightly different standards for rodent and nonrodent species. Briefly, the current requirements are as follows. Survival surgery may be conducted on rodents in an area that is used solely for this purpose while the surgery is being performed (such as a room or a portion of a room). The surgery must be performed using sterile instruments, surgical gloves, and aseptic procedures designed to prevent contamination of the operative site. In addition to the above requirements, survival surgery involving higher vertebrate species must use aseptic surgical techniques such as wearing sterile surgical gloves, gowns, caps, face masks; using sterile instruments; and preparing an aseptic surgical field. Furthermore a separate, dedicated surgical area, sub
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