WHAT SPIKING CONTROLS CAN I USE TO ESTIMATE CUSTOM ARRAY PERFORMANCE?
The factory-built controls from our standard factory layout can be used as spiking probes, providing that they do not cross-hybridize with your material. The sequences of these probes are shown in Table 1. You can order oligonucleotides that are reverse-complimentary to the selected control probes, and get them end-labeled (biotin or fluorescent dye, should be the same labeling as in the planned experiments). Two or three of these oligonucleotides would be sufficient as spiking controls to add to the hybridization mixture.
