What spectral range should be used for a good analysis?
Normally, one should use CD spectra at least in the range 190-240 nm. But sometimes experimental conditions limit the range to 200 nm or so, and the flexibility of the refernce sets in the more recent programs allows the use of 200-240 nm in CD analyses. The reliability, however, is slightly reduced as one reduces the wavelength range. Comparison of performances of the two spectral ranges are available in Sreerama and Woody. Anal. Biochem.
