What specific diseases can be diagnosed with PCR testing?
• Single PCR method. • Synbiotics research: 62 feline blood samples from Antech Labs were analyzed by ELISA, IFA and Synbiotics FeLV PCR. PCR had good correlation with strong positive IFA and strong positive ELISA. However, weak positive IFA conflicted with PCR. • 45 samples strong positive ELISA/42 PCR Positive/93% correlation. 5 samples weak positive ELISA/2 PCR Positive/40% correlation • PCR is helpful in resolving conflicts between antibody based tests. FIV (Feline Immunodeficiency Virus) • Double PCR Method. • Single PCR results with 12 antibody positive cats: 7 FIV PCR positive. • Double PCR on the same 12 cats: 12 PCR positive. • Note: Some cats fail to produce antibodies after infection, i.e., False Negative ELISA & Western Blots. False positive ELISA in kittens less than 6 months. • PCR is more sensitive for FIV detection–patent restrictions prohibit its commercial application. FIP (Feline Infectious Peritonitis) • RT-PCR (Reverse Transcription Polymerase Chain Reaction). • R
