What sort of resolution is possible using a single restriction enzyme?
The “resolution” of Optical Mapping using a single restriction enzyme is approximately 2Kb. Optical Mapping begins with immobilizing DNA to an optical glass surface. Because all wet chemistry occurs on this surface, very small fragments of the immobilized DNA can occasionally become detached and wash away. While small fragments may be retained that <2Kb in size, 100% will be retained that 2Kb and larger.
