What Solvents can be used with silica-based reversed phase columns?
• Silica-based reversed phase columns can be used with all common HPLC grade organic solvents. Buffers made from acetate, citrate, formate, and phosphate salts at concentrations up to 0.2M have been used without adverse effect. Organic modifiers and ion pair reagents also present no problems as long as the appropriate pH range is not exceeded. Because ion pair reagents are often difficult to completely flush from the column, columns used with these reagents should be dedicated to the particular analysis involved. Limit the use of strong acids and bases to amounts needed to adjust the pH of the mobile phase. Be careful not to mix, or use in sequence, solutions that might precipitate or gel in the column or system.
