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What size blot can I process in SNAP i.d.?

April 26, 2017blot process size snap

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What size blot can I process in SNAP i.d.?

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Blot holderMaximum Western Blot Single well7.5 x 8.4 Double well4.2 x 8.4 Triple well2.8 x 8.4 Q. Can I stack multiple blots in a blot holder well? • We do not recommend stacking blots within the blot holder as it will result in high signal variability. Q. What blotting membranes are compatibles with SNAP i.d. System? • All standard (nitrocellulose and PVDF) membranes are compatible. For optimal performance we recommend Immobilon-P membrane for chemiluminescent detection or Immobilon- FL membrane for fluorescent detection. Q. Can the SNAP i.d. System be used right after blot transfer? • Yes, as long as the blot is still wet. Q. Is any danger of drying the blot if the vacuum is applied for too long? • No, even if you keep the vacuum running for several minutes the blot will not dry since some liquid always remains within the blot holder. Blocking Q. Can I use non-fat dry milk (NFDM) for blocking in the SNAP i.d. System? • Yes, NFDM is excellent blocking reagent for the SNAP i.d. Detecti