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What sites should I use to transfer a TrueORF clone into the Gateway system?

April 26, 2017clone Gateway sites system transfer trueorf

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What sites should I use to transfer a TrueORF clone into the Gateway system?

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There are multiple sites in pCMV6-Entry than can be used to move the insert of a TrueORF clone into one of Gateway’s Entry vectors (pENTR-1A, -2B, -3C, -4, and -11). These sites are EcoRI, Sal I, BamHI and Kpn I at the 5’ end, and Not I at the 3’ end.