What sequencing redundancy is recommended?
This depends on the size of the organism you are trying to resequence. For whole-genome resequencing, a 25-fold over-sampling should be adequate. For targeted resequencing involving mixes of many PCR products, 75-fold over-sampling will correct for the inability to mix the PCR products at a 1:1 ratio. Illumina sample prep shows no systematic bias. In sequencing the X chromosome we achieved 16-fold average coverage, with all sequenceable bases covered at least twice. • What should I do if I get an error indicating that the program can’t be found? For example: -bash-3.1$ GAPipeline-1.3.2/bin/illumina2srf -o lane_1.srf / /Data/IPAR_1.3/Bustard1.3.2_01-03-2009/s_1_*_qseq.txt -bash: /GAPipeline-1.3.2/bin/illumina2srf: No such file or directory If you get this message, you should explicitly install the io_lib. To do this, run the following command from the Pipeline Install directory: /GAPipeline_1.3.2 make WITH_IO_LIB=1 install, then retry the command from Q2, above.
Related Questions
- Is full spacecraft redundancy a requirement for MIDEX? If not an explicit requirement, can it be inferred implicitly as a recommended architecture?
- Can Vimta prepare sequencing templates? What is the recommended method for template preparation?
- What does "essentially complete" correspond to in terms of sequencing redundancy?
