What sample collection methods should I follow to help minimize RNA degradation?
For researchers who are collecting tissue on their own, we suggest a few guidelines to limit RNA degradation and ensure sample quality. All samples submitted to the lab for extraction and downstream microarray applications should be handled in one of the following ways at the time of collection: • Tissue and cell pellets should be snap frozen in liquid nitrogen and then stored at -80�C until submitted to the CGF. • Tissue and cell pellets should be placed in RNAlater (Ambion, Inc) and stored at 4�C for up to one month or -20�C indefinitely until submitted to the CGF. • Tissue and cell pellets should be homogenized immediately in either Qiagen Buffer RLT (recommended- see link below) or Trizol (not recommended) and then stored at -80�C until submitted to the CGF. For more information on how to limit RNA degradation please see the following Tech Note provided by Ambion, Inc: http://www.ambion.com/techlib/tn/91/9113.html For product and ordering information for RNAlater: http://ww
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