What restriction enzymes should I use if Sgf I or Mlu I sites are present in my ORF?
While 96% of all human and mouse ORFs can use the Sgf I – Mlu I com¬bination, some ORFs do contain internal Mlu I site(s). Most of those ORFs with an internal Mlu I site can be transferred using another rare cutter (Rsr II), whose re¬striction site is upstream of Mlu I, or Not I, whose site is immediately downstream of Mlu I. Using one of the four different subcloning combinations, any ORF can be transferred from one vector to another. The recommended subcloning combina¬tion for every TrueORF cDNA is listed in the product information on our website.
