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What restriction enzymes are recommended for diagnostic digests of the clone DNA?

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What restriction enzymes are recommended for diagnostic digests of the clone DNA?

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We recommend Pvu II. Restriction digest with Pvu II results in three bands; however, clones with an additional Pvu II site in the hairpin insert will produce four bands: 2513 bp, 2325 bp, 1478 bp and 776 bp.

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