What quality control procedures are recommended when using the One-Direct System?
It is critical to run positive control amplifications using good quality RNA diluted to an appropriate level when working with lysates or small total RNA samples. It will frequently be impossible to determine the sample quality prior to running the amplification reaction due to sample size limitations. Amplification yield and cDNA fragment distribution can be important QC metrics to monitor. qPCR can also be a useful analysis tool for interrogating amplified cDNA.
