What protein concentration range is recommended prior to the start of labelling with CyDye™ DIGE Fluor minimal dyes?
Protein samples at higher concentrations (5–10 mg/ml) label more efficiently than dilute solutions (< 1 mg/ml). Protein concentration should not exceed 10 mg/ml as protein aggregation and precipitation may occur causing loss of protein and horizontal or vertical streaking (Gorg et al. The current state of two-dimensional electrophoresis with immobilised pH gradients. Electrophoresis 21, 1037–1053 [2000]). We recommend a concentration of 5 mg/ml for optimal labelling.
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