What procedures do you use to qualify antibodies for ChIP applications?
We qualify antibodies by using one of the two following ChIP assays: Query (QPCR): If binding sites are known (or proposed) for the factor of interest, small ChIP reactions using your samples are carried out to assess both absolute and relative signals at the known/proposed positive sites compared to negative genomic regions. ChIP-on-Chip: If no binding sites are known, a ChIP reaction using an appropriate chromatin sample is carried out followed by amplification and hybridization to a tiling array. The number and heights of the peaks are assessed to determine if the antibody works in ChIP.
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