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What primers may I use to sequence the shRNA cassette within the SureSilencing™ shRNA plasmids?

April 26, 2017cassette plasmids Primers sequence shrna suresilencing™

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What primers may I use to sequence the shRNA cassette within the SureSilencing™ shRNA plasmids?

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You may use the T7 and SP6, or the pUC/M13 forward and reverse primers to sequence the inserts of the SureSilencing™ shRNA plasmids. We recommend that sequencing be carried out in both directions.