What pools do you recommend for standardisation and positive controls of T-cell assays such as ELISA and CFC?
Both of our CEF pools (standard and extended) have been extensively used as positive, antigen specific control for CD8+ T cell stimulation in ELISPOT, intracellular cytokine and CTL assays. For efficiency in vitro stimulation of antigen-specific CD4+ and CD8+ T cells, we have developed a series of CMV related PepMixes such as the pp65-pool, the IE-1 pool, the IE-2 pool and others. For more information, refer to our web-page.
Both of our CEF pools (standard and extended) have been extensively used as positive, antigen specific control for CD8+ T cell stimulation in ELISPOT, intracellular cytokine and CTL assays. For efficiency in vitro stimulation of antigen-specific CD4+ and CD8+ T cells, we have developed a series of CMV related PepMixes such as the pp65-pool, the IE-1 pool, the IE-2 pool and others. For more information, see our references.
