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What packaging cell line should I use for making lentivirus?

April 26, 2017CELL lentivirus line packaging

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What packaging cell line should I use for making lentivirus?

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For packaging pGIPZ lentiviral shRNAmir constructs, the Trans-Lentiviral Packaging System is recommended by Open Biosystems. The Trans-Lentiviral Packaging System allows creation of a replication-incompetent, HIV-1-based lentivirus which can be used to deliver and express your gene or shRNA of interest in either dividing or non-dividing mammalian cells. The Trans-Lentiviral Packaging System uses a replication-incompetent lentivirus based on the trans-lentiviral system developed by Kappes, et al.