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What other acids can be used with the OMIX tips instead of TFA?

April 26, 2017acids omix tfa tips Used

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What other acids can be used with the OMIX tips instead of TFA?

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For analysis by LC/MS or LC/MS/MS, the TFA in the elution solvent only should be replaced with either 0.1% Formic Acid or 0.1% Acetic Acid to avoid ion suppression caused by TFA. Both the formic and the acetic acid can be used with a 50-75% acetonitrile solution for optimum recoveries. Note: to ensure maximum binding of peptides to the OMIX sorbent, the TFA solution should always be used in the pretreatment step.