What might be the reason for frost on chamber walls and the microtome?
• Poor air circulation around cryostat. • Sliding window seal is defective. Replace seal. • Locating the cryostat in an area where it is exposed to air currents (open windows or doors, overhead air vents, etc). A more suitable area should be found. • Leaving the sliding window of the cryostat open for an extended period. This can permit warm air from the room to enter the cryochamber and cause frost to form on the microtome and knife holder system. Ensure that the sliding window is closed after terminating work.
