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What method/protocol should I use to prepare total RNA for microarray analysis?

April 26, 2017analysis method microarray prepare Protocol RNA total

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What method/protocol should I use to prepare total RNA for microarray analysis?

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Although many methods exist for preparing total RNA, Affymetrix recommends RNA extraction using TRIzol reagent, followed by clean up with Qiagen RNeasy kits. Whichever method is used, the bottom line here is that pure, high quality RNA is needed to ensure good expression results.