WHAT METHOD DO I USE FOR QUALITY CONTROL OF CUSTOMARRAY™ MICROARRAYS AFTER STRIPPING?
The quality of stripping must be determined prior to re-hybridization. This is achieved by imaging the stripped microarray with the same high-resolution fluorescent microarray scanner and the same PMT gain settings as used for the first hybridization and imaging of the microarray. Do not increase the PMT gain setting, as this may increase the background fluorescence.