What may be the cause of a cell line not growing well on NUNC Brand products?
Numerous factors influence cell attachment and growth on cell culture vessels. Poor cell attachment can be due to one or more of the following: • Environmental culture conditions such as temperature, pH osmolality (concentration of various salts in buffer), humidity and the overlying gas phase (CO2 and O2 concentration). • Contamination by microbial agents (bacteria, yeast and molds). Antibiotics are used to minimize the risk of these contaminants. Cell cultures can be contaminated without visible signs of a problem. Viruses and mycoplasma are the most common. Detection of mycoplasma is often difficult. Serum should be obtained from a supplier who certifies it to be free of mycoplasma. • Components in the medium could be toxic to some cells lines. • Wash detergent residues left on laboratory glassware may affect cell attachment. Detergent residues may be toxic, in effect solulabilizing the cell membrane or cellular proteins. • Some cells require an extracellular matrix substrate such a
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