What lysis buffer should I use to extract protein?
We recommend using 2-D lysis buffer (7 M urea, 2 M thiourea, 4% CHAPS, 30 mM Tris-HCl, pH 8.8) for protein extraction. If other buffers are used, you can replace the starting buffer with 2-D lysis buffer, or you can send us the protein extract, and we will exchange the buffer for 2-D lysis buffer here (free of charge). We prefer the protein concentration to be around 2 to 8 mg/ml. Samples in 2-D lysis buffer can be stored at -80 C for many months.
