What liquid scintillation counter window settings do I need to use for counting an SPA assay?
A. For counting SPA assays in a liquid scintillation counter or instruments other than the Packard TopCount or Wallac Microbeta, set the windows to wide open or count in a P channel. Make certain that you use a blank such as beads plus radioactive tracer, as well as any other assay-specific controls to assess origins of any detectable background.