What kinds of proteins have been synthesized in the wheat germ cell-free expression system?
CellFree Sciences Co., Ltd and Ehime University have collectively synthesized more than 6,000 proteins, which include nuclear receptors (e.g., ER), transcriptional factors (e.g., NF-κB), Ser/Thr kinases (p38α, CaMK, etc.), Tyr kinases (JAK, TIE2, etc.), proteases (caspases, SARS protease, etc.), pathogenic proteins (e.g., malaria plasmodium proteins), and others. Not subjected to species limitation, the wheat germ cell-free expression system has been used to synthesize a broad range of proteins, from proteins of microorganisms (bacteria, thermophiles, pathogens, etc.) to those of higher forms of life (plants, fish, and mammals). Certain membrane proteins have been synthesized in a soluble form, if yields are low.
Related Questions
- What are the advantages of the wheat germ expression system compared with other systems for synthesizing recombinant proteins?
- Can a different promoter other than SP6 be used for the wheat germ cell-free expression system?
- What kinds of proteins have been synthesized in the wheat germ cell-free expression system?
