What kind(s) of antigen(s) can be injected?
Immunization can be done using native proteins, recombinant proteins, peptides carbohydrates or other compounds of microbial, fungal or virus origin. Minimum molecular weight needed to induce sufficient immune response is from 5 – 10 kDa. Biohazardous materials for immunizations are not accepted. Important notes: • If antibodies are going to be used on the denatured target protein (example:Western blot, immunohistochemistry on fixed tissues), denatured forms of antigens are preferred (like gel piece, protein in inclusion bodies). • If antibodies are going to be used on native target proteins (example: immunoprecipitation), non denatured forms of antigen are preferred (protein in solution free of denaturating agents). • Not all anti-peptide antibodies will recognize native protein, therefore a careful choice of peptide sequence is of crucial importance. • Antibodies made against recombinant proteins expressed in bacteria can in some cases fail to recognize native protein. The reason for
