What kind of quality control is carried out on oligonucleotides?
Top Our Oligonucleotides are synthesized by the highest quality equipment and reagents to guarantee excellent results. Every oligo will go through high quality QC by MALDI-TOF (mass spectroscopy). MALDI-TOF MS (Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectroscopy) is an analytical method that allows the detection of the composition of various biological components, such as peptides and oligonucleotides. An oligonucleotide is embedded in a matrix on a metal surface and ionized into gas phase within the machine chamber by the energy of laser beam. In the gas phase, the ionized oligonucleotides accelerate towards the detector, which determines the masses according to the time of flight. The yield of every oligonucleotide is accurately determined and if it does not meet our standards the oligonucleotide is re-synthesised. We have comprehensive checks at every stage during production and strict quality assurance policies. 13. I used an oligonucleotide in a cloning ex
