What kind of material can be analyzed?
Fresh cells (in suspension or adherent) or fresh tissues can be used for nuclear protein extraction. As the TFs are highly conserved during their evolution, the TF Chip Stem Cell Kit can analyze material from human, rat and mouse. The TF Chip MAPK Kit can analyze material from human and mouse. Please keep in mind that the workflow in the protocol for extraction of nuclear proteins depends on the cells / tissues that shall be applied to the TF chips.