What kind of HPLC purification is performed?
When HPLC purification is ordered, several methods of HPLC purification could be used. For unmodified oligos, ion exchange purification is often performed as this method typically provides the best separation of full length oligos from the lesser products. On certain modified oligos we use reversed-phase HPLC as the modification increases the hydrophobicity of the modified versus unmodified oligos providing the best separation for these products.. Two-step HPLC purification, which includes reversed-phase HPLC followed by ion exchange HPLC, is recommended for oligos longer than 70 bases.
