What kind of data has been used to update ORF predictions between Wormbase versions 9 and 100?
Wormbase curators mainly used EST, OST, cDNA and RNA data to curate the C. elegans genome. Other factors such as comparative genomics, SL sequences, Blast results or the overlap of a predicted ORF with a newly annotated non-coding region or a modified neighboring gene helped reannotate the C. elegans genome. Comparative genomics certainly represents the most promising genome annotation tool for C. elegans in the near future. Although only one additional nematode, C. briggsae, has been sequenced so far, the sequences of 3 additional nematode species is underway and will represent a powerful predictor of C. elegans ORF structures.
