What kind of controls are included in the DualChip microarray kit?
1. Internal standard mix – spike-in control, has to be added to the cDNA synthesis reaction – can be used as positive control for the cDNA synthesis and for normalization of the results – can be used for normalization of generated ratios – In the case of two-color analysis it also allows the evaluation of dye bias 2. Pre-labeled positive hybridization controls (biotinylated PCR product) has to be added to the hybridization solution – can be used to control hybridization efficiency 3. Positive biotin detection control (probe spotted on the array) – can be used to control biotin detection efficiency 4. Negative hybridization control (probe spotted on the array) – to monitor the specificity of the assay 5. Negative detection control (probe spotted on the array) – to monitor the specificity of the detection process.
