What is Tyramide Signal Amplification (TSA™)?
Tyramide signal amplification is a novel technology developed and patented by PerkinElmer scientists. It is an extremely powerful signal amplification technology that can be used in many applications including immunohistochemistry, in situ hybridization, electron microscopy, ELISA, and others. It can be used with both chromogenic and fluorescence visualization methods. Additional advantages of the tremendous boost in signal from the TSA reagents include the potential to reduce the use of valuable reagents or improve signal to noise by reducing the titer of a problematic reagent in the assay protocol. Use of TSA facilitates multi-target detection in both IHC and (F)ISH applications. The TSA reagents can be easily added to any standard IHC protocol. For (F)ISH applications, standard nonradioactive DNA, RNA, and oligo probe labeling procedures can be used for probe generation, using common nonradioactive labeling haptens such as biotin, fluorescein, and digoxigenin. After standard hybridi
