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What is the Xi-Clone PCR Cloning System?

April 26, 2017cloning PCR system xi-clone

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What is the Xi-Clone PCR Cloning System?

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The Xi-Clone PCR Cloning System is a unique and powerful cloning system that allows you to save days of time by cloning your genes of interest into high expression Xi-Clone vectors without using restriction or ligation enzymes. You simply mix your PCR amplified gene with a linearized Xi-Clone vector, and then add this mix to the SmartCells Competent E. coli (included with the Xi-Clone kit). After the transformation is complete, > 85% of the transformants contain the insert in the correct orientation.