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What is the value of a confocal image?

April 26, 2017confocal image value

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What is the value of a confocal image?

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A confocal image has the out-of-focus haze removed. This can theoretically increase image resolution. The increase in resolution, by as much as 1.4 times (Brackenhoff, 1989), results in improved measurements (Yelamarty, 1990) and visualization. In addition, the optical sectioning is non-invasive and can be performed on living specimens. Also, it is possible to acquire images with multiple wavelengths of light and merge the results for greater information. Besides increasing the resolution of the image, the deconvolved slices can be stacked to produce a three-dimensional representation of the specimen. Visualization of a three-dimensional data set can lead to new insights.