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What is the usual cell density/volume we are working with for the mouse splenocyte BD Phosflow protocols?

April 26, 2017BD CELL density mouse phosflow protocols usual volume working

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What is the usual cell density/volume we are working with for the mouse splenocyte BD Phosflow protocols?

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Depending upon the experiment, we use around 4 million cells per mL, in a volume of 2 mL for stimulation. We recommend performing timepoints (for example, 5, 10, 15 minutes) to find the optimal stimulation conditions. We recommend optimizing activation conditions for your application.