WHAT IS THE TYPICAL ERROR RATE ASSOCIATED WITH USAGE OF HYB & SEQ RESEQUENCING? HOW DO REGIONS OF SECONDARY STRUCTURE AFFECT HYB & SEQ PERFORMANCE?
Hyb & Seq demonstrates 90-100% accuracy with re-sequencing. Most errors seem to be associated with secondary structure such as hairpins and palindromes. When hairpins and palindromes are present in the single-strand target DNA, they tend to self-hybridize within the target sequence, rather than hybridize to the corresponding probes on the array. The result will be a decreased signal for that portion of the sequence.
