What is the total yield (yield per use, uses per spin column, and total yield per kit) of partitioned proteins using the IgY-14 and HSA spin columns?
The serum/plasma loading capacity of the IgY12 spin column is 10 µL. Assuming a total serum/plasma protein range of 70-80 mg/mL, one can load 700-800 µg protein on the IgY12 spin column. About 90% of the total protein is bound to the IgY-14 column, yielding an eluted quantity of 720 µg. About 10% of the total protein is present in the flow-through fraction, yielding a quantity of about 80 µg with the 12 most abundant proteins removed. Since each spin column can be used 100 times, the total yield is ~8 mg of partitioned protein. Since a kit contains 2 spin columns, each IgY12 Spin Column kit should yield a total of about 16 mg of IgY12-partitioned protein.
