Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

What is the subtype of the Ad5 viral backbone used for theAdeno-Quest constructs?

April 26, 2017backbone constructs subtype Used viral

0

Posted

What is the subtype of the Ad5 viral backbone used for theAdeno-Quest constructs?

1 Answer

0

Posted

A. The viral backbone we use for the Adeno-Quest constructs is an Ad5 subtype delta L327, deleted in a XbaI fragment of 1.89 kb that covers almost all of the E3 region from 28,593-30,471 (78.5-84.3 Map units). The backbone is further E1 deleted from nucleotides 358-3,332 (numbers are based on Ad5 wt sequence, map units 1.4 to 9.4). This removes nearly all the E1 sequences between the viral packaging domain and the coding sequence for pIX.